The plant biostimulants (PBs) are a wide range of microbial and/or organic compounds applied to crops to improve the physiological processes such as nutrition efficiency, plant development and abiotic stress tolerance. Imazamox is a herbicide characterised with a wide spectrum of weed control, low application rates and low mammalian toxicity, but also with a high soil persistence. Therefore, the residual amounts of imazamox may negatively affect subsequent sensitive crops in the crop rotation. In the current study we investigated the effect of a single and combined treatment with imazamox and a plant biostimulant (protein hydrolisate) on the antioxidative defense system and the detoxification metabolism of wheat young plants. The result showed that the seed imbibition with 10 μM imazamox inhibits the growth of the young wheat plants. A slight improvement was found due to the additional treatment with protein hydrolysate of the wheat plants damaged by imazamox herbicide. According to the results, this improving effect on the growth does not ameliorate the plant detoxification metabolism such as glutathione S-transferases or antioxidative defense. However, the improving effect is low and insufficient to restore the plant growth and functioning and its effects on wheat production are not studied yet.