Abstract
Previous studies have analyzed bacterial taxa associated with rhizospheric cinnamon forest soil. Cicer montbretii Jaub. & Spach (C. montbretii), an endemic species for the region of Strandzha, was analyzed using a metagenomic approach. The diversity of endophytic microorganisms is essential for ecology, as it makes it possible to understand in detail the plant-microbial interactions. The objectives of this study were to isolate and identify endophytic bacteria from the roots of C. montbretii associated with cinnamon forest (A1) and yellow earth podzolic soils (A2). A study on endophytic bacteria in the roots of C. montbretii legume plant, done by assessing its PCR amplicon of 16S rDNA with the Illumina metagenomics technique, used to generate a total of 81,782 reads from the samples. The analyses revealed that Proteobacteria were similar in cinnamon forest and in yellow earth podzolic soils. Bacteriodota prevailed in yellow earth podzolic soils. Firmicutes and Actinobacteria were the common genera in cinnamon forest soil. The roots of the plants grown in A1 soil had the largest composition with Proteobacteria 78%, which consisted of the genus— Erwinia 13%, Pseudomonas 11%, Shingomonas 11%, Rhizobium 5%, and Bradirhyzobium 4%. In the A2 soil prevailed Proteobacteria 76% and Bacterioidota 15%. The most abandoned genus in A2 was Orchobactrum 20%, Stenotrophomonas 15%, Sphingobacterium 12%, Serratia 4%, and Flavobactrerium 4%. The α-diversity analysis indicated the richness and inverse Simpson diversity index of the bacterial endophyte communities for the root tissues to be 6.551 for A1 and 4,692 for A2 soil, respectively. The endophytic bacterial communities differed among the two soil samples, indicating that the soil and the climatic conditions considerably affected the entire endophytic community.
Keywords: NGS, Endophytes, Cicer montbretii, Strandzha Nature Park