Potatoes are a valuable source of carbohydrates, vitamins and minerals and therefore have a particular significance to human diet. Different varieties suitable for cooking, baking, preparation of chips, etc., have been developed, which are characterized with specific biochemical composition of the tubers. Apart from the methods of selection, the technology of cultivation and in particular the applied fertilization approaches also influence the quality of the crop yield. This is especially important, since the current worldtrend is high-quality food production with minimal negative impact on the environment. The utilization of organic fertilizers is one of the prerequisites for achieving that goal. Moreover, the requirements for high quality are valid also when the production should be stored for certain periods. Therefore, the aim of the current study is to investigate the influence of different rates of fertilization with vermicomposton the content of ascorbic acid and monosaccharides in potatoes from the Agria variety. The experiments were performed with fresh production and with tubers stored under different conditions. The potatoes were grown in the fields in four variants, each with three replication and every replication on 10 m2. The variants included: a control with non-treated plants and 3 fertilization rates – 50, 100 and 150 ml per cluster, respectively. The fertilizer was applied once during planting. The experimental results demonstrated that the highest content of ascorbic acid and monosaccharides was observed in the fresh samples treated with 100 ml fertilizer per cluster. On the other hand, a change in the concentrations of those metabolites was found after storage at 200C and 60C.